Unfortunately some bromine 82 also distributes into the cells. Extracellular fluid volume measurement.

Measurement Of Fluid Volumes In The Different Body Fluid
Measurement of body fluid volume. The plasma volume is calculated by evans blue technique the proportion of plasma and hematocrit is by centrifugation. To be a perfect marker a substance must also not be metabolised. Thus bromine measurements of the ecf volume frequently over estimate the ecf volume. Measuring body fluid compartments. Water is necessary for all life on earth. Not interfere with body fluid distribution if the tracer is excreted in the urine then the loss can be determined and corrections made in the calculation.
Euvolemia is the state of normal body fluid volume including blood volume interstitial fluid volume and intracellular fluid volume. If the tracer is metabolised a series of measurements can be made and assuming exponential decline first order kinetics the volume of distribution can be determined by extrapolation back to zero time. Hypovolemia and hypervolemia are imbalances. To measure the volume of any fluid compartment within the body you must inject or infuse a marker substance that will equilibrate diffuse freely to a uniform concentration throughout this compartment. Humans can survive for 4 to 6 weeks without food but only for a few days without water. Then make proportion calculation.
If you measure too soon all you will get is the volume of the functional extracellular fluid. Measuring the extracellular fluid volume with crystalloid tracer. Body fluid compartments renal physiology lecture 1 part 1 duration. If plasma is x and its volume in body is y then plasmahematocrit100 the volume in body is v. Volume measurement of compartments usmle step 1 dr.